01 lab Skills

MY EXPERIMENTAL ABILITIES

MOLECULAR BIOLOGY

Cloning, seamless cloning, vector development, qPCR

PROTEIN TECHNIQUES

Protein purification (FPLC), westen blot and gel electrophoresis

EXPRESSION SYSTEM

Chlamydomonas reinhardtii, Escherichia coli and Pichia pastoris

DESIGN OF EXPERIMENTS

Full factorial design, composite central design

HARDWARE DEVELOPMENT

Tubular photobioreactor, microcentrifuge and cell counter

Example

BIOTECH RESEARCHER & TINKERER

I'M

João Vitor.

I WOULD LIKE TO INVITE YOU TO EXPLORE MY WORK, SKILLS AND PROFESSIONAL EXPERIENCE IN THIS PAGE. I'M PASSIONATE ABOUT SCIENCE AND ALL ITS FACETS. I STRONGLY BELIEVE IN THE CAPACITY OF HUMAN INGENUITY TO OVERCOME OBSTACLES, SPECIALLY IN RESEARCH. THIS BELIEVE LEAD TO TACKLE MY RESEARCH INTERESTS IN A MULTIDISCIPLINARY APPROACH; FROM DEVELOPING PLASMID VECTORS TO LAB EQUIPMENTS, AND BIOPROCESS. TAKE A MOMENT TO LOOK AROUND ON MOLINOJVD WebSite, AND CONTACT ME DIRECTLY TO LEARN MORE.

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ABOUT ME

Production of recombinant proteins in microalgae
Microalgae is a promising platform to produce recombinant proteins. It grows in simple and cheap media, with eukaryotic machinery to produce complex proteins. We developed plasmid vectors for the production of recombinant proteins, as well as, equipments.

PhD in Biochemical and Pharmaceutical Technology - USP

2013-2017

Technical trainee

The project was part of the program Trust in Science of GSK. The project focused in identifying new drug candidates to Chagas disease from synthetic derivatives and from computational docking and virtual screening.

GlaxoSmithKline/USP

2012

Researcher
Stephen Mayfield Lab`s in San Diego has long tradition in recombinant microalgae and technology transferring to industry. I got the opportunity to be part of the lab and observe the development of high tech startups.

Visiting Scholar, UCSD

2014-2015

03 Experience

Product Development Scientist

Development of interference RNA to Agrotech applications. Silencing technology applied to high efficiency and low environmental impact, in the fight against pests in agriculture.

LOTAN AgroSciences

2017-2018

Extraction of Adenovirus in aqueous two-phase system

Aqueous two phase system are a mild extraction strategy to recover from small chemical molecules to entire cells. They are composed mostly of water, and can be formed with the combination of polymers, and salts or surfactants. We developed a system to extract Adenovirus particles from crude lysate, while removing cell debris.

Master in Biochemical and Pharmaceutical Technology - USP

2010-2012

Undergrad internship

The University of Campinas (UNICAMP) was bestowed in a Biotech pole in Brazil, with several research labs. I enrolled in lab activities in the university to work with biomarkers in E.coli. Outside the campus, I endeavor to develop marker to assisted selection of coffee plants.